| Mallory’s
Iron Staining Procedure
Literature
Purpose:
This standard operating procedure (SOP) establishes the standard
for staining iron pigment using the Mallory’s Iron staining
procedure.
Reagents
and Materials
Reagents
5%
Potassium Ferrocyanide
Potassium
Ferrocyanide 25gm
Distilled water 500ml
5% Hydrochloric Acid
Hydrochloric
Acid 25ml
Distilled water 475ml
Make
fresh:
5%
Hydrochloric Acid 20ml
5% Potassium Ferrocyanide 20ml
Nuclear Fast Red
Commercially
prepared by Newcomer Supply Co. (catalog # 12552)
Expiration date provided by manufacturer.
Store at room temperature.
Materials
Tissue slides and control slides for special stain requested.
Microwave to bake slides.
Staining rack, 20 slide capacity.
Coverslips and synthetic mounting media.
Hazards
and Precautions:
See Material Safety Data Sheets.
Sample Preparations:
Paraffin tissue section slides cut at 4 microns.
Calibration
and Standardization:
A tissue section slide containing iron pigment is stained with each
procedure.
Procedure:
1. Deparaffinize and hydrate to distilled water.
2. Iron Stain - 10 minutes.
Make
fresh:
5% Hydrochloric Acid 20ml
5% Potassium Ferrocyanide 20ml
3.
Distilled water rinse.
4. Nuclear Fast Red - 5 minutes.
5. Distilled water rinse.
6. Dehydrate, clear and coverslip.
Positive control: dog spleen with hemosiderosis
Results:
Iron pigments - bright blue
Nuclei – dark pink to red
Cytoplasm - light pink
Cherie
Chapman
James R. Turk
7/1/04
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